proteome analysis of rat hippocampus following morphine-induced amnesia and state-dependent learning

Authors

saeideh jafarinejad-farsangi department of cell and molecular biology, school of biology, college of science, university of tehran, tehran, iran

ali farazmand department of cell and molecular biology, school of biology, college of science, university of tehran, tehran, iran

ameneh rezayof department of animal biology, school of biology, college of science, university of tehran, tehran, iran

niloufar darbandic department of biology, faculty of science, arak university, arak, iran

abstract

morphine’s effects on learning and memory processes are well known to depend on synaptic plasticity in the hippocampus. whereas the role of the hippocampus in morphine-induced amnesia and state-dependent learning is established, the biochemical and molecular mechanisms underlying these processes are poorly understood. the present study intended to investigate whether administration of morphine can change the expression level of rat hippocampal proteins during learning of a passive avoidance task. a step-through type passive avoidance task was used for the assessment of memory retention. to identify the complex pattern of protein expression induced by morphine, we compared rat hippocampal proteome either in morphine-induced amnesia or in state-dependent learning by two-dimensional gel electerophoresis and combined mass spectrometry (ms and ms/ms). post-training administration of morphine decreased step-through latency. pre-test administration of morphine induced state-dependent retrieval of the memory acquired under post-training morphine influence. in the hippocampus, a total of 18 proteins were identified whose mascot (modular approach to software construction operation and test) scores were inside 95% confidence level. of these, five hippocampal proteins altered in morphine-induced amnesia and ten proteins were found to change in the hippocampus of animals that had received post-training and pre-test morphine. these proteins show known functions in cytoskeletal architecture, cell metabolism, neurotransmitter secretion and neuroprotection. the findings indicate that the effect of morphine on memory formation in passive avoidance learning has a morphological correlate on the hippocampal proteome level. in addition, our proteomic screen suggests that morphine induces memory impairment and state-dependent learning through modulating neuronal plasticity.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Proteome Analysis of Rat Hippocampus Following Morphine-induced Amnesia and State-dependent Learning

Morphine’s effects on learning and memory processes are well known to depend on synaptic plasticity in the hippocampus. Whereas the role of the hippocampus in morphine-induced amnesia and state-dependent learning is established, the biochemical and molecular mechanisms underlying these processes are poorly understood. The present study intended to investigate whether administration of morphine ...

full text

Proteome Analysis of Rat Hippocampus Following Morphine-induced Amnesia and State-dependent Learning

Morphine’s effects on learning and memory processes are well known to depend on synaptic plasticity in the hippocampus. Whereas the role of the hippocampus in morphine-induced amnesia and state-dependent learning is established, the biochemical and molecular mechanisms underlying these processes are poorly understood. The present study intended to investigate whether administration of morphine ...

full text

Proteome Analysis of Rat Hippocampus Following Morphine-induced Amnesia and State-dependent Learning

Morphine's effects on learning and memory processes are well known to depend on synaptic plasticity in the hippocampus. Whereas the role of the hippocampus in morphine-induced amnesia and state-dependent learning is established, the biochemical and molecular mechanisms underlying these processes are poorly understood. The present study intended to investigate whether administration of morphine ...

full text

Research Paper: Cross State-dependent Learning Interaction Between Scopolamine and Morphine in Mice: The Role of Dorsal Hippocampus

Introduction: The current study aimed at investigating the existence of the cross state-dependent learning between morphine and scopolamine (SCO) in mice by passive avoidance method, pointing to the role of CA1 area.  Methods: The effects of pre-training SCO (0.75, 1.5, and 3 μg, Intra-CA1), or morphine (1, 3, and 6 mg/kg, intraperitoneal (i.p.) was evaluated on the retrieval of passiv...

full text

Morphine sensitization and state-dependent learning in mice

In the present study, the effects of morphine sensitization on morphine-induced impairment of memory formation and the state-dependent retrieval of a passive avoidance task learned under morphine influence have been investigated in mice. Pre-training administration of morphine (0.5, 2.5 and 5 mg/kg) dose dependently suppressed the learning of one-trial passive avoidance task. Pre-test administr...

full text

Morphine sensitization and state-dependent learning in mice

In the present study, the effects of morphine sensitization on morphine-induced impairment of memory formation and the state-dependent retrieval of a passive avoidance task learned under morphine influence have been investigated in mice. Pre-training administration of morphine (0.5, 2.5 and 5 mg/kg) dose dependently suppressed the learning of one-trial passive avoidance task. Pre-test administr...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of pharmaceutical research

جلد ۱۴، شماره ۲، صفحات ۵۹۱-۶۰۲

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023